Download PDF by Neil Raymond Sims, Patrick Robert Carnegie (auth.), B. W.: Advances in Neurochemistry: Volume 3

By Neil Raymond Sims, Patrick Robert Carnegie (auth.), B. W. Agranoff, M. H. Aprison (eds.)

ISBN-10: 1461582407

ISBN-13: 9781461582403

ISBN-10: 1461582423

ISBN-13: 9781461582427

The unique premise of the Editors in beginning this sequence was once that there existed a readership ofneurochemists with huge biochemical again­ flooring who might utilize a sequence devoted to either new strengthen­ ments and really expert experiences in neurochemistry. Having chosen our authors, we now have provided them nearly entire freedom to mirror and speculate in a box during which they've got accomplished prominence. The reaction to the 1st volumes has been worthwhile. the current one keeps during this culture. whereas we've not tried to submit really good volumes, the current quantity includes a little similar chapters (Chapters four and five, at the position of amino acid neurotransmitters). the 1st 3 chapters study 3 diversified techniques, each one of present curiosity, in neurochemi­ cal ways to the molecular bases of neuronal and glial constitution. B. W. Agranoff M. H. Aprison vii CONTENTS bankruptcy 1 2',3'-CYCUC NUCLEOTIDE 3'-PHOSPHODIESTERASE NEIL RAYMOND SIMS AND PATRICK ROBERT CARNEGIE 1. creation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2. Assay of CNPase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . three 2. 1. desire for Activation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . three 2. 2. comparability of Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . three three. organization of CNPase with Myelin . . . . . . . . . . . . . . . . . . . . . . . 7 three. 1. historic. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 three. 2. Subcellular Fractionation. . . . . . . . . . . . . . . . . . . . . . . . . . . . eight three. three. improvement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . eight three. four. Mutant Mice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . nine three. five. Use as a Myelin Marker . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 three. 6. Myelin-Related Fractions and Peripheral Nerve Myelin. eleven four. CNPase in Nonmyelin Fractions. . . . . . . . . . . . . . . . . . . . . . . . . . thirteen five. Activation and Isolation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . thirteen five. 1. creation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . thirteen five. 2. Activation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 five. three. Solubilization and Fractionation. . . . . . . . . . . . . . . . . . . . . . 15 6. houses. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 6. 1. Substrate Specificity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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Detection of myelin deficiency is usually achieved by comparing CNPase levels in homogenates of unfractionated material obtained from test and control groups, a simple approach which has proven satisfactory in the majority of cases. Small lesions of demyelination or those localized to a narrow region of the brain may sometimes pass undetected although this can often be overcome by determining levels for different regions of the CNS rather than the brain as a whole, and by selecting likely areas from the clinical and histological presentation.

Katzman, and B. W. ), pp. 365-386, Little, Brown, Boston, Mass. Norton, W. , 1977, Isolation and Characterization of myelin, in: Myelin (P. ), pp. 161-199, Plenum Press, New York. Norton, W. , Poduslo, S. , 1966, Subacute sclerosing leukoencephalitis, II. Chemical studies including abnormal myelin and an abnormal ganglioside pattern, J. Neuropathol. Exp. Neural. 25:582-597. Olafson, R. , Drummond, G. , and Lee, J. , 1969, Studies on 2' ,3 '-cyclic nucleotide-3'phosphohydrolase from brain, Can. J.

Neurol. Sci. 25: 19-27. Volpe, J. , Marasa, J. , and Agrawal, H. , 1975, Relation ofC-6glial cells in culture to myelin, Biochem. J. 152:701-703. Waehneldt, T. , 1975, Ontogenetic study of a myelin-derived fraction with 2' ,3 '-cyclic nucleotide 3'-phosphohydrolase activity higher than that of myelin, Biochem. J. 151:435437. Waehneldt, T. , 1972, Isolation of rat brain myelin, monitored by polyacrylamide gel electrophoresis of dodecyl sulfate-extracted proteins, Brain Res. 40:419-436. Wassenaar, J.

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Advances in Neurochemistry: Volume 3 by Neil Raymond Sims, Patrick Robert Carnegie (auth.), B. W. Agranoff, M. H. Aprison (eds.)


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